Did you know that the amount of cuddles a baby receives from a caregiver could affect the baby’s DNA methylation profile, and in turn, the developmental progress?1 In fact, DNA methylation affects almost every facet of daily life.2 But, what is DNA methylation?
DNA methylation is a chemical modification to DNA molecules. In most cases, DNA methylation refers to the covalent addition of a methyl group to the 5-carbon position of cytosine, forming 5-methylcytosine (5mC).
DNA methylation is the most extensively studied epigenetic phenomena, as well as the most naturally occurring.3 In eukaryotes, DNA methylation pervasively occurs at CpG dinucleotides. The patterns of DNA methylation at promoters, gene bodies and enhancers play vital roles in regulating gene expression.4-6 DNA methylation affects gene expression by typically silencing genes through the process of adding methyl groups to specific DNA regions, which prevents transcription factors from binding and accessing the gene, thus inhibiting its transcription and protein production. Essentially, highly methylated DNA is usually associated with decreased gene expression while unmethylated DNA is more readily accessible for transcription.
Some DNA methylation patterns have been widely studied, and researchers have acquired a fair amount of knowledge on their regulation of gene expression. A few examples of such DNA methylation patterns are as follows:
Given that precise gene regulation is important for many fundamental biological processes, DNA methylation is essential throughout organismal development and homeostasis. The applications of DNA methylation assessment vary from cancer, aging, metabolic health to forensic testing.
The gold-standard reaction for DNA methylation analysis is called bisulfite conversion. Bisulfite reagents convert unmethylated cytosine residues to uracils (as illustrated in Figure 2) while leaving the methylated ones untouched.
Many methods have been developed to investigate DNA methylation-based on bisulfite conversion. Since the demand of genome-wide DNA methylation profiling has increased, NGS-based methods have become much more popular.
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Benefits of Next-Generation Sequencing (NGS)
Bisulfite sequencing has gained tremendous popularity among researchers for DNA methylation profiling, indicated by the increasing number of related publications over the years. DNA samples that are bisulfite converted must be constructed into libraries that contain adapters for sequencing on a compatible NGS instrument. The methylated cytosines are recognized as “C” in NGS readouts.
Thus far, researchers have implemented a myriad of bisulfite sequencing methods to uncover DNA methylation information for diverse biological questions.
The most extensively used NGS methods include reduced representation bisulfite sequencing (RRBS), whole-genome bisulfite sequencing (WGBS), and targeted bisulfite sequencing.
These methods share common steps in the procedure of library preparation such as bisulfite conversion, adapter ligation and PCR amplification, but differ slightly at the beginning of the workflows during the preparation of the input DNA materials.
Interested in learning more about these methods, their differences, and which one best suits your research? Select your preferred NGS methods below to find out more!
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